Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to ... The Cas9 nuclease can be delivered as mRNA or protein; the single guides are ... Try our Guide-it Recombinant Cas9 (Electroporation-Ready) for your ...
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guide it recombinant cas9 electroporation ready user manual
by EC Freund · 2020 · Cited by 5 — Both plasmid transfection and viral transduction methods have been developed for ... As with the individual guides, the standard and XT gRNA pools ... of recombinant Cas9 permits pooled functional screening in myeloid cells ... gDNA extracts were stored at −20°C until ready for use as PCR templates.. Reference guide: Perform gene knockout with the Alt-R CRISPR-Cas System » · Reference ... Alt-R CRISPR-Cas9 System—RNP electroporation, Amaxa Nucleofector ... Recombinant S. pyogenes Cas9 nuclease, purified from an E. coli strain ... the Alt-R User Guides, using Alt-R CRISPR-Cas9 guide RNAs (crRNA:tracrRNA .... by A Elaswad · 2018 · Cited by 8 — In this protocol, guide RNAs and Cas9 protein were microinjected into ... The main alternative method for microinjection is electroporation, ... lectin (RBL) gene and prepared according to Shah et al. with the use ... Cas9 protein, PNA Bio Inc. CP01, Recombinant Cas9 protein from Streptococcus pyrogenes. 939c2ea5af
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